Plant Tissue

Preparation of the sample using the following protocol:

• Glutaraldehyde Fixation Classic - Osmium - Included in Epon
• Contrast the UranyLess monitoring Lead Citrate

Phage T6

• Staggering Phage T6 on a G300-Cu grid Covered with a Carbon Formvar Film. Ionization 1 minute

Liver Mouse and Gerbil Sahara

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess Lead - Citrate

Culture Cells, Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - Contrast UranyLess lead -citrate

Muscle - Nerve - Mice

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute

PLC Contrast Leica EM Stain

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - contrast Uranyless Lead -Citrate

Chantal Cazevieille CRIC / IURC INSERM Montpellier tested aqueous UranyLess in the Leica brand grid contrast controller on different tissues, Drosophila heart atrium, retina, cochlea and ileum (Gut). The tissues were fixed according to the standard protocol 2.5% Glutaraldehyde in PHEM buffer, the post fixation in 0.5% osmium in 0.8% potassium ferrocyanide in RT for 2 hours. The sections are collected on single-hole or 200 mesh grids.
The treatment of the grids is UranyLess 7mn lead citrate followed 7 minutes. We present here some images made by Hitachi transmission electron microscope with a digital camera AMT. You will notice that the combined action of potassium ferrocyanide and UranyLess reveal a marked way the cyto-membranes in the ileum.

Mouse Kidney

Preparation of the sample using the following protocol:

• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
• Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute

Mouse Cardiac Muscle

Preparation of the sample using the following protocol:

• Classic - Fixing Glutaraldehyde, Osmium, Epon Ultrafine
• Cups, Double UranyLess Contrast and Lead Citrate

Negative staining with Uranyless examined by TEM