Uranyl Acetate Replacement Stain (UAR-EMS)

Description

UAR Stain is a new negative stain which may be used as an alternative to Uranyl Acetate. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Not recommended as a fixative.

How to use Uranyl Acetate Replacement Stain

The UAR Stain is supplied as a concentrate. Please use the following dilutions:

• For most samples, including, but not limited to, virus', dilute by 4x in distilled water
• If you are working with Bacillus, then a dilution of 15-40x is needed.

Staining will take place in 30 minutes at room temperature. Post fixation with Osmium Tetroxide recommended for high-contrast staining. For increased contrast of the stain, simply adjust the staining time.

Masamichi Nakakoshi, Hideo Nishioka and Eisaku Katayama (2011) New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate, Journal of EIectron Microscopy 60(6 ): 401-407