Fluoro-Gel II with DAPI, Mounting Medium

Fluoro-Gel II is similar to Fluoro-Gel, but Fluoro-Gel II contains DAPI (4, 6-diamino-2-phenylindole), which is a counter stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing blue fluorescence. RNA is also stained with DAPI.

Instructions for use

1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
3. Turn the vial upside down and open the dropper to remove any air bubbles.
4. Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
5. Let stand at room temperature for about 5 minutes.
6. Apply cover slip carefully avoiding air bubbles.
7. One can seal the edges of cover slip with nail polish, any organic medium or a Limonene mounting medium (E17987-01). If a coverslip is not sealed air bubbles will appear in few days.
8. For storage it is recommended that the slide be stored in the dark at 2-8°C.

Handling and storage
This product can be store at room temp, however for long term storage 2-8°C is recommended. Protect from light, DO NOT FREEZE

Removal of coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.