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DER 332-732

Three different mixtures: one for relatively soft blocks, another for harder blocks for collagenous tissue, and a tougher one if required.

Grouped product items
Product Name Qty
DER 332-732 Epoxy Embedding Kit

E14000

€154.20 €183.50
DER 732 Resin, 225ml

E13010

€49.10 €58.43
DER 332 Resin, 225ml

E13030

€49.10 €58.43
D.D.S.A., Dodecenyl Succinic Anhydride, Specially Distilled, 450ml

E13710

€84.20 €100.20
BDMA, Benzyldimethylamine, 100ml

E11400

€52.60 €62.59
DMP-30, 2,4,6-Tri(Dimethylaminomethyl)-Phenol, 25ml

E13600

€15.20 €18.09

Product Details

Description

Introduction

This kit combines two epoxy resins, DER 332, a very pure and uniform epoxy having a low viscosity, and DER 732, a polyglycol diepoxide which imparts softness and flexibility to the polymers of conven-tional epoxides, but does not cause shrinkage or serious loss of strength. Together with their curing agents, this kit produces a standard amber-colored block which can be trimmed and sectioned easily.

Recommended Procedure

Fixation: Tissues can be fixed in a wide range of fixatives. One of the more commonly used fixatives is an alde-hyde (i.e.: glutaraldehyde) followed by osmium tetroxide.

Dehydration: There are many different dehydration schedules that can be followed. A typical one is as follows:

  • 70% Ethanol for 10 minutes
  • 100% Ethanol for 10 minutes100% Ethanol for 15 minutes
  • 100% Propylene Oxide for 15 minutes
  • 100% Propylene Oxide for 15 minutes

**NOTE: Longer times may be required for some samples.

Mixing Instructions:

Components Softer Harder Tougher, Soft A/E Ratio*
DER 332 7m 7ml 6ml 7ml
DER 732 3ml 2ml 3ml 3.2ml
DDSA 5ml 5ml 10ml 8ml
DMP 30 0.3ml 0.28ml 0.38ml 0.3ml

(FOR LARGER BATCHES INCREASE EACH COMPONENT PROPORTIONALLY)

Slight variations of the accelerator (DMP-30) will drastically affect the color and brittleness of the block.

Prior to measuring and mixing, the resin and the anhydride should be warmed (60°C) to reduce their viscosity. Thorough mixing is imperative to be able to achieve uniform blocks.

Although the mixture can be stored for up to 6 months at 4°C it is highly recommended that freshly prepared embedding medium always be used. If you choose to store the mixture you should warm it thoroughly prior to adding the accelerator.

Infiltration:It is recommended that for all of the infiltration steps a specimen rotator be used.

  • Drain the tissue of most of the propylene oxide, leaving a little so the tissue does not dry out.
  • Replace the solvent with a 1:1 solution of propylene oxide:embedding medium and allow it to stand for at least 1 hour at room temperature.
  • Remove the mixture, replace it with 100% embedding medium and leave for 6-12 hours at room temperature.

Embedding:This may be done in embedding capsules #E70020 or a flat embedding mold #E70900).

Transfer each sample to a dry capsule or mold and fill the mold with embedding medium. Curing of the medium may be carried out in an oven at 37°C, 45°C, and 60°C for 24 hours at each temperature.

Blocks can be trimmed and sectioned after the blocks return to room temperature.

*This formula was suggested by Bluemink(1970) in accordance with the recommendations of Coulter(1967) concerning anhydride/epoxy ratios.

References
Lockwood, W.R.(1964), Anatomical Record, 150, 129.Bluemink, J.G.(1970), J. Ultrastruct. Res. 32, 142.

More Information

More Information
Application
Embedding
Base
Epoxy
Mandatory sign
Tightly sealed goggles Tightly sealed goggles
Protective gloves Protective gloves
Signal word
Warning
Symbol GHS
GHS07 GHS07
Component Parts 2x225ml DER 332, 225ml DER 732, 450ml DDSA, 50ml DMP-30/BDMA
Shipping Class
Hazardous Good LQ
Storage Temperature
RT
SDS (multilingual) E13030, E13010
SDS (multilingual) E13710
SDS (multilingual) E13600
SDS (multilingual) E11400