CitiFluor™ Tris-MWL 4-88, Mounting Medium, 25ml

Description

Citifluor Tris-MWL 4-88 is a classical, popular mountant based on proprietary poly (vinyl alcohol), MowiolTM 4-88. The composition of the solution is well tried and tested and contains the polymer, glycerol and tris-amine buffer. It has a pH of ~8.5. It can be used as a hardening mountant on its own, but if antifadent properties are required, it should be used with either AF200 or AF300.

Properties and storage of MWL 4-88 solutions

The solutions are of medium viscosity, are water-white in appearance and have a pH of ~8.5. They may be stored at room temperature. The cap of the bottles should always be replaced after use, to prevent evaporation of water which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration e.g. no gel formation.

Usage and Useful Tips

The solution is of medium viscosity and has a pH of ~8.5 and contains a small amount of sodium azide to prevent microbial growth.

Following loss of water it forms good clear films and is therefore used as a permanent mountant.

Citifluor Tris-MWL 4-88 itself has very little antifadent properties. To change the mountant into one with antifadent properties it should be used with preferably AF200 and AF300 antifadent solution.

A few drops of the solution should be applied to the specimen followed by a cover slip. The water evaporates to give a clear film which holds the cover slip in place thereby aiding the safe storage of the specimen.

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