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CitiFluor™ AF87, Mounting Medium
Citifluor AF87 is an immersion oil and mounting medium with high refractive index and low auto-fluorescence containing an antifading/anti-bleaching agent. The high refractive index is ideal for examination of specimens with confocal laser scanning microscopy (CLSM).
Refractive index at 20°C: 1.516
Product Details
Description
Description
AF87 is a non-fluorescent immersion oil of medium viscosity and has a refractive index of 1.516 and can be used as an immersion oil or as a mountant medium.
Properties and storage of AF87
The immersion oil should be stored at 5° to 10°C and should be kept out of strong sunlight. It should NOT be stored in a refrigerator. Occasionally small crystals are formed in the oil. Careful use of a pipette can obviate the crystals interfering with the function of the oil.
Usage and Useful Tips
Usage as immersion oil
It is recommended that the microscope objective is wiped clean after use. A useful solvent mixture for removing the immersion oil is composed of ether/ethanol (7:3 v/v) or alternatively, xylene may be used.
Usage as mountant medium
It is necessary to dehydrate the sample prior to application by washing it with successive amounts of absolute ethanol prior to drying. Once the sample is dry the AF87 can be applied. The AF87 contains an antifadent to reduce the amount of fluorochrome fading. AF87 has been found useful for examining specimens generated using the technique of FISH, labelled with the fluorochromes DAPI and Cy dyes.
For more information please see our Info-Box.
More Information
| Mandatory sign |
Tightly sealed goggles
|
|---|---|
| Signal word |
Danger
|
| Symbol GHS |
GHS07
GHS08
|
| Hazard statements |
H302
H304
H315
H319
H336
H413
|
| Precautionary statements |
P501
|
| ready to use | yes |
| Used for Fluorescence |
yes
|
| Manufacturer |
EMS
|
| SDS (multilingual) | E179760-10, E17976-25 |
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